Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume 13, Issue 34, Pages 7980-7986Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.2c01692
Keywords
-
Categories
Funding
- National Science Foundation (NSF) [TG-MCA05S027, MCB-1716956, CNS-1725797]
- Frontera Grants [MCB21002, MCB20034]
- National Institutes of Health (NIH) [R01-GM118560-01A]
- Czech Science Foundation [20-24155S, 22-15374S]
- [A1_FCHI_2022_002]
Ask authors/readers for more resources
This study investigated the solvation thermodynamics of proteins in two osmolyte solutions using various experimental and simulation techniques. It was found that existing force fields were unable to accurately capture the solvation properties of proteins and led to unphysical denaturation. By developing a new force field and introducing appropriate scaling, the experimental solution properties were successfully reproduced and the unphysical denaturation was prevented.
Using a combination of molecular dynamics simulation, dialysis experiments, and electronic circular dichroism measurements, we studied the solvation thermodynamics of proteins in two osmolyte solutions, trimethylamine N-oxide (TMAO) and betaine. We showed that existing force fields are unable to capture the solvation properties of the proteins lysozyme and ribonuclease T1 and that the inaccurate parametrization of protein-osmolyte interactions in these force fields promoted an unphysical strong thermal denaturation of the trpcage protein. We developed a novel force field for betaine (the KBB force field) which reproduces the experimental solution Kirkwood-Buff integrals and density. We further introduced appropriate scaling to protein-osmolyte interactions in both the betaine and TMAO force fields which led to successful reproduction of experimental protein-osmolyte preferential binding coefficients for lysozyme and ribonuclease T1 and prevention of the unphysical denaturation of trpcage in osmolyte solutions. Correct parametrization of protein-TMAO interactions also led to the stabilization of the collapsed conformations of a disordered elastin-like peptide, while the uncorrected parameters destabilized the collapsed structures. Our results establish that the thermodynamic stability of proteins in both betaine and TMAO solutions is governed by osmolyte exclusion from proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available