Identification and characterization of major alkaloid from Sinomenium acutum stem and their metabolites after oral administration in rat plasma, urine, bile and feces based on UPLC-Q-TOF/MS

Sinomenium acutum stem is widely used to treat rheumatoid arthritis, gout, ankylosing spondylitis and other diseases in China. However, its metabolism in vivo is still unclear. In this study, UPLC-Q-TOF/MS was used to analyze the main components and their metabolites in rats after oral administration of Sinomenium acutum stem extract. A total of 41 compounds were identified from the ethanol extract of Sinomenium acutum stem based on the established database and the reference substance; a total of 25 prototype components and 107 metabolites (74 phase I metabolites and 33 phase II metabolites) were speculated and identified in the plasma, urine, bile and feces of rats administered. The metabolic pathways included hydroxylation, demethylation, dehydrogenation, glucuronidation and acetylation. In conclusion, this study revealed the metabolism of Sinomenium acutum stem in vivo, which may provide a better basis for the study of Sinomenium acutum stem and provide useful chemical information on the material basis and pharmacological mechanism of drug efficay.

Automated summary

This study used UPLC-Q-TOF/MS to analyze the main components and their metabolites of Sinomenium acutum stem in rats. The study identified 41 compounds, including 25 prototype components and 107 metabolites. The metabolic pathways involved hydroxylation, demethylation, dehydrogenation, glucuronidation, and acetylation. This study provides important insights into the metabolism of Sinomenium acutum stem and its pharmacological mechanism.