Quantifying Shear-induced Margination and Adhesion of Platelets in Microvascular Blood Flow

Platelet margination and adhesion are two critical and closely related steps in thrombus formation. Using dissipative particle dynamics (DPD) method that seamlessly models blood cells, blood plasma, and vessel walls with functionalized surfaces, we quantify the shear-induced margination and adhesion of platelets in microvascular blood flow. The results show that the occurrence of shear-induced RBC-platelet collisions has a remarkable influence on the degree of platelet margination. We characterize the lateral motion of individual platelets by a mean square displacement analysis of platelet trajectories, and find that the wall-induced lift force and the shear-induced displacement in wall-bounded flow cause the variation in near-wall platelet distribution. We then investigate the platelet adhesive dynamics under different flow conditions, by conducting DPD simulations of blood flow in a microtube with fibrinogen-coated wall surfaces. We find that the platelet adhesion is enhanced with the increase of fibrinogen concentration level but decreased with the increase of shear rate. These results are consistent with available experimental results. In addition, we demonstrate that the adherent platelets have a negative impact on the margination dynamics of the circulating platelets, which is mainly due to the climbing effect induced by the adherent ones. Taken together, these findings provide useful insights into the platelet margination and adhesion dynamics, which may facilitate the understanding of the predominant processes governing the initial stage of thrombus formation.(c) 2022 Elsevier Ltd. All rights reserved.

Automated summary

Platelet margination and adhesion in thrombus formation were investigated using dissipative particle dynamics (DPD) method. The shear-induced RBC-platelet collisions were found to significantly affect platelet margination. The lateral motion of individual platelets and platelet adhesive dynamics were characterized, revealing the impact of wall-induced lift force, shear-induced displacement, and fibrinogen concentration on platelet distribution and adhesion. The findings provide insights into the initial stage of thrombus formation.