4.3 Article

Detecting aquatic pathogens with field-compatible dried qPCR assays

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 202, Issue -, Pages -

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ELSEVIER
DOI: 10.1016/j.mimet.2022.106594

Keywords

Lyophilization; qPCR; Air-dried assay; Environmental diagnostics; Aquatic pathogens; eDNA

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Field-ready qPCR assays with extended shelf-life are developed and tested for monitoring emerging aquatic pathogens, enabling quick conservation and management decisions.
Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.

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