Journal
CARBOHYDRATE POLYMERS
Volume 128, Issue -, Pages 63-74Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.04.010
Keywords
Green synthesis; Hyaluronic acid; Gold nanoparticles; Metformin; Zebrafish; HepG2 cells
Categories
Funding
- University Grants Commission (UGC), India [F1-17.1/2011-12/RGNF-SC-TAM-2228/(SA-III)]
- Department of Science Technology
- Department of Biotechnology
Ask authors/readers for more resources
In this study, green synthesis of gold nanoparticles (AuNPs) was achieved using the extract of eggplant as a reducing agent. Hyaluronic acid (HA) serves as a capping and targeting agent. Metformin (MET) was successfully loaded on HA capped AuNPs (H-AuNPs) and this formulation binds easily on the surface of the liver cancer cells. The synthesized nanoparticles were characterized by UV-Vis spectrophotometer, HR-TEM, particle size analyser and zeta potential measurement. Toxicity studies of H-AuNPs in zebra fish confirmed the in vivo safety of the AuNPs. The in vitro cytotoxicity results showed that the amount of MET-H-AuNPs enough to achieve 50% inhibition (IC50) was much lower than free MET. Flow cytometry analysis showed the significant reduction in G2/M phase after treatment with MET-H-AuNPs, and molecular level apoptosis were studied using western blotting. The novelty of this study is the successful synthesis of AuNPs with a higher MET loading and this formulation exhibited better targeted delivery as well as increased regression activity than free MET in HepG2 cells. (C) 2015 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available