Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 58, Issue 8, Pages 749-758Publisher
WILEY-BLACKWELL
DOI: 10.1111/jipb.12467
Keywords
AGO1; functional complementation; maize; ZmAGO1a
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Funding
- National Natural Science Foundation of China [31361140364, 31171562]
- National High Technology Research and Development Program of China [2012AA10A306]
- Agricultural Science and Technology Innovation Program (ASTIP) of CAAS
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Eukaryotic Argonaute proteins play primary roles in miRNA and siRNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four ZmAGO1 genes have not yet been characterized in maize (Zea mays L.). In the present study, ZmAGO1a was identified from four putative ZmAGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with ZmAGO1a indicated that constitutive overexpression of ZmAGO1a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype. The expression profiles of ZmAGO1a under five different abiotic stresses indicated that ZmAGO1a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat. Further, variation in ZmAGO1a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus. The present data suggest that ZmAGO1a might be an important AGO1 ortholog in maize. The results presented provide further insight into the function of ZmAGO1a.
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