Journal
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
Volume 98, Issue 3, Pages 322-333Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14620316.2022.2128442
Keywords
Pogostemon cablin; VIGS; PTS; patchouli alcohol; sesquiterpene metabolism
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In this study, a VIGS system was established for functional characterization of candidate genes in Pogostemon cablin. Silencing of PDS and PTS genes successfully suppressed the biosynthesis of patchouli alcohol and other compounds.
In plant species, virus-induced gene silencing (VIGS) effectively enables the transient knock down of target genes for functional analysis. To overcome the bottleneck preventing the functional genomics and biological engineering of Pogostemon cablin (Blanco) Benth. an important medicinal and industrial plant with high-value natural products, the VIGS system was established to enable the fast and efficient functional characterisation of candidate genes. In our study, the effectiveness of phytoene desaturase (PDS) gene silencing by VIGS was monitored by the visual indications in P. cablin and the activation effect spanned several months. VIGS of the PatPTS gene, encoding patchoulol synthase, caused a decline in expression abundance, resulting in a significant decreasing accumulation of patchouli alcohol and other sesquiterpenes. Cosilencing of PatPDS and PatPTS in P. cablin also suppressed the biosynthesis of patchouli alcohol. Negative feedback of the MVA and MEP pathway genes was demonstrated in PatPTS-silenced plants.
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