Nanoplastics cause transgenerational toxicity through inhibiting germline microRNA mir-38 in C. elegans

Nanoplastic exposure potentially caused the induction of transgenerational toxicity. Nevertheless, the molecular basis for nanoplastic exposure-induced transgenerational toxicity remains largely unclear. Using Caenorhabditis elegans as an animal model, we examined the role of germline microRNA (miRNA) mir-38 in regulating the transgenerational toxicity of polystyrene nanoparticles (PS-NPs). After the exposure, 1-100 mu g/L PS-NP decreased expression of germline mir-38. Meanwhile, germline mir-38 overexpression conferred a resistance to transgenerational PS-NP toxicity, which suggested that the decrease in germline mir-38 mediated the induction of transgenerational PS-NP toxicity. In the germline, mir-38 regulated transgenerational PS-NP toxicity by inhibiting activity of downstream targets (NDK-1, NHL-2, and WRT-3). Among these three downstream targets, germline NDK-1 further controlled transgenerational PS-NP toxicity by suppressing the function of KSR-1/2, two kinase suppressors of Ras. Therefore, in the germline, the decrease in mir-38 mediated induction of trans generational PS-NP toxicity by at least inhibiting signaling cascade of NDK-1-KSR-1/2 in nematodes. The findings in this study are helpful for providing relevantly molecular endpoints to assess potential transgenerational toxicity of nanoplastics.

Automated summary

Nanoplastic exposure may induce transgenerational toxicity, with germline microRNA mir-38 playing a role in regulating the toxicity of polystyrene nanoparticles.