Simultaneous elimination of antibiotics and antibiotics resistance genes in nitritation of source-separated urine

Antibiotics in human urine could accelerate dissemination of antibiotics resistance genes (ARGs), posing potential threat to sewage. The nitritation of source-separated urine was a critical step to realize the urine resourcelization and nitrogen stabilization. However, the synergic control on antibiotics and ARGs during urine nitritation was unrevealed. This study investigated the removal profiles of five typical antibiotics and the shifts of microbial community and ARGs during stable nitritation. The result showed that sulfamethoxazole and roxithromycin were effectively eliminated with high removal efficiency of (95 +/- 5) % and (90 +/- 10) %, followed by enrofloxacin with removal efficiency of (60 +/- 5) %, whereas trimethoprim and chloramphenicol showed low removal efficiency of less than 40 %. Ammonia oxidation bacteria and heterotrophic bacteria equally contributed to elimination of sulfamethoxazole with a high biodegradation rate of 0.1534 L/gVSS.h, while sorption and biodegradation jointly promoted other antibiotics removal. The total relative abundance of top 25 bacteria genera was decreased by 10 %. The total relative abundance of top 30 ARGs was decreased by more than 20 %, which was corresponding to the variation of bacterial community. The findings in this research would get a deeper insight into the eliminating antibiotics and controlling ARGs dissemination during nitritation of source-separated urine.

Automated summary

This study investigated the removal profiles of antibiotics and antibiotic resistance genes (ARGs) during urine nitritation. The results showed that sulfamethoxazole and roxithromycin were effectively eliminated, while trimethoprim and chloramphenicol had low removal efficiency. Ammonia oxidation bacteria and heterotrophic bacteria played important roles in sulfamethoxazole degradation.