Journal
CARBOHYDRATE POLYMERS
Volume 118, Issue -, Pages 41-43Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.10.074
Keywords
Oligosaccharides; Flammulina velutipes; Hydrogen peroxide
Categories
Funding
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2). The yields of F. velutipes derived oligosaccharides (FVOs) were monitored during the hydrolysis process. FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70 degrees C, H2O2 concentration 3%, and reaction time 6h. Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method. The oligosaccharides were partially characterized by Fourier transform infrared spectrum, monosaccharide composition, and antioxidant activity. The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 mu g/mL. (C) 2014 Elsevier Ltd. All rights reserved.
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