Journal
CARBOHYDRATE POLYMERS
Volume 121, Issue -, Pages 449-456Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.12.058
Keywords
Ephedra sinica; Arabinan; NMR; Structural elucidation
Categories
Funding
- Major State Basic Research Development Program of China (973 Programs) [2013CB531800]
- National Natural Science Foundation of China [81274103, 30973870, 81303215]
- Foundation for the Author of National Excellent Doctoral Dissertation of PR China [201367]
- Heilongjiang Postdoctoral Foundation [LBH-Q13158, LBH-Z10019]
- Foundation from Heilongjiang University of Chinese Medicine [035094]
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Plant arabinan has important biological activity. In this study, a water-soluble arabinan (M-w similar to 6.15 kDa) isolated from the stems of Ephedra sinica was found to consist of (1 -> 5)-Araf, (1 -> 3,5)-Araf, T-Araf, (1 -> 3)-Araf and (1 -> 2,5)-Araf residues at proportions of 10:2:3:2:1. A tentative structure was proposed by methylation analysis, nuclear magnetic resonance (NMR) spectroscopy (H-1 NMR,C-13 NMR, DEPT-135, H-1-H-1 COSY, HSQC, HMBC and ROESY) and literature. The structure proposed includes a branched (1 -> 5)-alpha-Araf backbone where branching occurs at the O-2 and O-3 positions of the residues with 7.7% and 15.4% of the 1,5-linked alpha-Araf substituted at the O-2 and O-3 positions. The presence of a branched structure was further observed by atomic force microscopy. This polymer was characterized as having a much longer linear (1 -> 5)-alpha-Araf backbone as a repeating unit. In particular, the presence of alpha-Araf -> 3)-alpha-Araf-(1 -> 3)-(alpha-Araf-(1 -> attached at the O-2 is a new finding. This study may facilitate a deeper understanding of structure-activity relationships of biological polysaccharides from the stems of E. sinica. (C) 2014 Elsevier Ltd. All rights reserved.
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