Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 298, Issue 10, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jbc.2022.102468
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Funding
- Intramural Research Program of the National Cancer Institute, National Institutes of Health
- [GM62116]
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This study has identified 26 anti-glycan antibodies, most of which bind microbial carbohydrates, and found that these antibodies predominantly originate from IgG+ memory B cells, rather than from naive, early emigrant, or immature B cells.
The immune system produces a diverse collection of anti-glycan antibodies that are critical for host defense. At present, however, we know very little about the binding properties, origins, and sequences of these antibodies because of a lack of access to a variety of defined individual antibodies. To address this challenge, we used a glycan microarray with over 800 different components to screen a panel of 516 human mono-clonal antibodies that had been randomly cloned from different B-cell subsets originating from healthy human sub-jects. We obtained 26 antiglycan antibodies, most of which bound microbial carbohydrates. The majority of the antiglycan antibodies identified in the screen displayed selective binding for specific glycan motifs on our array and lacked poly-reactivity. We found that antiglycan antibodies were about twice as likely than expected to originate from IgG+ memory B cells, whereas none were isolated from naive, early emigrant, or immature B cells. Therefore, our results indicate that certain B-cell subsets in our panel are enriched in antiglycan antibodies, and IgG+ memory B cells may be a promising source of such antibodies. Furthermore, some of the newly identified anti-bodies bound glycans for which there are no reported mono-clonal antibodies available, and these may be useful as research tools, diagnostics, or therapeutic agents. Overall, the results provide insight into the types and properties of antiglycan antibodies produced by the human immune system and a framework for the identification of novel antiglycan antibodies in the future.
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