Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 214, Issue -, Pages S319-S325Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiw270
Keywords
Ebolavirus; selective autophagy; ER-phagy; reticulon; FAM134B; mouse models; virus replication
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Funding
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
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Selective autophagy of the endoplasmic reticulum (termed ER-phagy) is controlled by members of the FAM134 reticulon protein family. Here we used mouse embryonic fibroblasts from mice deficient in FAM134B to examine the role of the ER in replication of historic (Mayinga) or contemporary (Makona GCO7) strains of Ebola virus (EBOV). Loss of FAM134B resulted in 1-2 log(10) higher production of infectious EBOV, which was associated with increased production of viral proteins GP and VP40 and greater accumulation of nucleocaspid lattices. In addition, only 10% of wild-type cells contained detectable nucleoprotein, whereas knockout of FAM134B resulted in 80% of cells positive for nucleoprotein. Together, these data suggest that FAM134B-dependent ER-phagy is an important limiting event in EBOV replication in mouse cells and may have implications for further development of antiviral therapeutics and murine models of infection.
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