Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 70, Issue 34, Pages 10563-10570Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c04651
Keywords
Hyphozyma roseonigra; biotransformation; sclareol glycol; sclareol; intermediates; whole-cell biocatalysis
Funding
- Natural Science Foundation of Guangxi [2021GXNSFBA196087, 2021AC19176, 2018GXNSFBA281017]
- Science Foundation Project of Guangxi Academy of Sciences [2020YBJ703, 2021YFJ1210, CQ-D-2415]
- National Natural Science Foundation of China [21868007]
- Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS- QYZD-201]
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This study investigates the dynamic process of sclareol biotransformation and proposes a potential transformation pathway of sclareol in Hyphozyma roseonigra based on detected intermediates.
Sclareol glycol is a key starting material with significant market interest for synthesizing high-value ambroxide, a sustainable substitute for ambergris in high-end fragrances. Sclareol glycol can be obtained by biotransformation of sclareol, a labdane-type diterpene, using Hyphozyma roseonigra. However, the pathway and mechanism of sclareol glycol biosynthesis remain unclear. In this study, the dynamic time course of sclareol biotransformation was explored by resting cell assays and several intermediates produced during biotransformation were detected. The results show that (1) sclareol glycol and sclareolide are not interconverted and are potentially synthesized via different metabolic pathways and (2) several putative intermediates resulting from biotransformation are featured with a labdane carbon backbone, including isomerized and oxidized analogues. A plausible transformation pathway of sclareol in H. roseonigra was proposed based on detected metabolites. This study sheds light on the biosynthetic mechanism of sclareol glycol and paves a way for the future biotechnological production of this promising compound.
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