Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume -, Issue -, Pages -Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c05593
Keywords
polysaccharide; large yellow tea; structure characterization; macrophage polarization
Funding
- National Natural Science Foundation of China [U19A2034]
- China Agriculture Research System of MOF and MARA ( [CARS-19]
- Introduction and Stabilization of Talent Projects of Anhui Agricultural University [rc352107]
- Natural Science Projects for Colleges and Universities in the Anhui Province [KJ2021A0140]
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In this study, a novel homogeneous polysaccharide (LYP-S3) that promotes the M2 polarization of macrophages was obtained from large yellow tea. The structural characterization revealed its composition and backbone. In vitro bioactivity evaluation showed that LYP-S3 has potential functions in preventing inflammation and obesity by reducing M1 macrophage markers and increasing M2 macrophage markers.
A novel homogeneous polysaccharide (LYP-S3) that promotes the M2 polarization of macrophages was obtained from large yellow tea by a bioactivity-guided sequential isolation procedure and activity evaluation in the present study. Structural characterization revealed that LYP-S3 has an average molecular weight of 28.6 kDa and is composed of rhamnose, arabinose, galactose, glucose, and galacturonic acid at the molar ratio of 8.08:11.66:11.77:3.96:58.02. The main backbone of LYP-S3 consists of -> 4)-alpha-D-GalpA-6-OMe-(1 ->, beta-D-GalpA-(1 ->, -> 4)-beta-D-Galp-(-> 1, and ->beta-D-Galp-(1 ->, and the branches are composed of alpha-L- Araf-(-> 1, -> 5)-alpha-L-Araf-(1 ->, -> 2,4)-beta-L-Rhap-(1 ->, -> 2)-beta-L-Rhap-(1 ->, and -> 4)-beta-D-Glcp-(1 ->. An in vitro bioactivity evaluation assay showed that LYP-S3 remarkably reduced the expression of M1 macrophage markers and increased the expression of M2 macrophage markers. In addition, LYP-S3 inhibited adipocyte differentiation and adipogenesis in 3T3-L1 adipocytes and blocked macrophage migration toward 3T3-L1 adipocytes in the cocultures of bone-marrow-derived monocytes and 3T3-L1 adipocytes. Furthermore, LYP-S3 promoted the M2 polarization of macrophages in cocultures. These findings suggested that LYP-S3 has a potential function in preventing inflammation and obesity.
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