Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 70, Issue 43, Pages 13959-13968Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c05667
Keywords
immobilized glucose isomerase; mVBA-b-P(AAm-co-AN); UCST-responsive polymers; fructose
Funding
- National Natural Science Foundation of China
- [22078133]
- [22278191]
- [21878131]
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This study developed a novel recyclable mVBA-b-P(AAm-co-AN)pglucose isomerase biocatalyst with improved substrate affinity and catalytic efficiency. Compared to the free enzyme, this biocatalyst showed superior stability and recyclability, making it a potential candidate for industrial production of high-fructose syrup.
The development of a suitable immobilization strategy to improve the performance of immobilized glucose isomerase for the isomerization of glucose to fructose is crucial to promoting the industrial production of high-fructose syrup. In this work, a novel recyclable upper critical solution temperature (UCST)-type mVBA-b-P(AAm-co-AN)pglucose isomerase biocatalyst (PVAA@GI) was prepared, and the immobilized glucose isomerase could capture the glucose substrate through the affinity of 4-vinylbenzeneboronic acid (4-VBA) and the glucose substrate, which led to the enhanced substrate affinity and catalytic efficiency of the PVAA@GI. The biocatalyst exhibited excellent stability in pH, thermal, storage, and recycling compared to the free enzyme. The mVBA-b-P(AAm-co-AN)pi glucose isomerase biocatalyst displayed reversibly soluble-insoluble characteristics with temperature change, which was in the soluble state during the enzyme reaction process but could be recovered in an insoluble form by lowering the temperature after the reaction. The highest fructose production rate reached 62.79%, which would have potential application in the industrial production of high-fructose syrup.
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