Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 70, Issue 39, Pages 12700-12707Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c04548
Keywords
logic gate; pesticide; acetamiprid; atrazine; CRISPR-Cas12a; intelligent detection
Funding
- Guangdong Basic and Applied Basic Research Foundation [2021A1515012192]
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An intelligent detection platform based on CRISPR-Cas12a and signal amplification circuits was developed for the detection of pesticides in food samples. The platform exhibited simple operation, high sensitivity, and multiple logic functions.
An intelligent detection platform was developed through molecular logic gate operation based on CRISPR-Cas12a and signal amplification circuits using two kinds of pesticides [acetamiprid (ACE) and atrazine (ATR)] as inputs. The pesticide- aptamer bindings activate the signal amplification process to produce numerous double-stranded DNA, which can be identified by CRISPR-Cas12a. Under the optimal assay conditions, the sensor exhibits excellent analytical performance, with the detection limits for ACE and ATR of 2.5 and 0.2 pM, respectively. The practicality of the platform was verified by testing pesticide concentrations in food samples. Several molecular logic gates (OR, AND, XOR, and INHIBIT) were constructed using 0 and 1 to encode the target pesticides and the fluorescence readout. The logic detection platform with simple operation, high sensitivity, and multiple logic functions is promising to become a powerful sensing system for the intelligent assay of different pesticides in food samples.
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