Actinomadura spongiicola sp. nov., isolated from the marine sponge Leucetta chagosensis

A novel actinomycete strain, named LHW52907(T), was isolated from a marine sponge (Leucetta chagosensis) collected in the South China Sea. The strain developed branched mycelia without fragmentation and short spore chains in hook - and-spiral form with wrinkled surfaces, bearing no more 10 spores. The cell -wall hydrolysates contained meso- diaminopimelic acid as the diagnostic diamino acid. The sugars in whole -cell hydrolysates consisted of mannose, ribose, glucose, galactose and madurose. The major fatty acids of the strain were C-16:0, C-17:0 and C-18:1 omega 9c. The predominant menaquinone was MK-9(H-6). The strain had the highest 16S rRNA gene sequence similarity of 99.72 % to Actinomadura pelletieri DSM 43383(T). However, the average nucleotide identity and in silico DNA-DNA hybridization values between them were 93.6 and 52.6 %, respectively, readily distinguishing them as two different species. The results indicate that strain LHW52907(T) represents a novel species of the genus Actinoma-dura, for which we propose the name Actinomadura spongiicola sp. nov, with the type strain LHW52907(T) (=DSM 106571(T)=CGMCC 4.7596(T)).

Automated summary

A novel actinomycete strain, Actinomadura spongiicola sp. nov, was isolated from a marine sponge collected in the South China Sea.