Journal
INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume 17, Issue -, Pages 4619-4638Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S379526
Keywords
photodynamic therapy; oxygen generation; magnetic resonance imaging; photoacoustic imaging; theranostics; nanoprobe
Funding
- National Natural Science Foundation of China
- Shandong Provincial Key Research and Development Project
- Science and Technology Planning Projects of Shandong Provincial Education Department
- [81641074]
- [11805247]
- [2018YFJH0501]
- [J17KB082]
Ask authors/readers for more resources
A novel multifunctional nano-snowflake probe (USPIO@MnO2@Ce6, UMC) was developed for oxygen-enhanced photodynamic therapy. The probe efficiently degraded under high H2O2 levels in tumor cells, accelerating the production of ROS during PDT. TEM and UV-vis spectroscopy results confirmed successful synthesis of UMC, with in vitro and in vivo experiments demonstrating its imaging capabilities and enhanced treatment efficiency compared to free Ce6.
Introduction: Accurate tumor diagnosis is essential to achieve the ideal therapeutic effect. However, it is difficult to accurately diagnose cancer using a single imaging method because of the technical limitations. Multimodal imaging plays an increasingly important role in tumor treatment. Photodynamic therapy (PDT) has received widespread attention in tumor treatment due to its high specificity and controllable photocytotoxicity. Nevertheless, PDT is susceptible to tumor microenvironment (TME) hypoxia, which greatly reduces the therapeutic effect of tumor treatment.Methods: In this study, a novel multifunctional nano-snowflake probe (USPIO@MnO2@Ce6, UMC) for oxygen-enhanced photodynamic therapy was developed. We have fabricated the honeycomb-like MnO2 to co-load chlorin e6 (Ce6, a photosensitizer) and ultrasmall superparamagnetic iron oxide (USPIO, T1-T2 double contrast agent). Under the high H2O2 level of tumor cells, UMC efficiently degraded and triggered the exposure of photosensitizers to the generated oxygen, accelerating the production of reactive oxygen species (ROS) during PDT. Moreover, the resulting USPIO and Mn2+ allow for MR T1-T2 imaging and transformable PAI for multimodal imaging-guided tumor therapy.Results: TEM and UV-vis spectroscopy results showed that nano-snowflake probe (UMC) was successfully synthesized, and the degradation of UMC was due to the pH/ H2O2 responsive properties. In vitro results indicated good uptake of UMC in 4T-1 cells, with maximal accumulation at 4 h. In vitro and in vivo experimental results showed their imaging capability for both T1-T2 MR and PA imaging, providing the potential for multimodal imaging-guided tumor therapy. Compared to the free Ce6, UMC exhibited enhanced treatment efficiency due to the production of O2 with the assistance of 660 nm laser irradiation. In vivo experiments confirmed that UMC achieved oxygenated PDT under MR/PA imaging guidance in tumor-bearing mice and significantly inhibited tumor growth in tumor-bearing mice, exhibiting good biocompatibility and minimal side effects.Conclusion: The multimodal imaging contrast agent (UMC) not only can be used for MR and PA imaging but also has oxygenenhanced PDT capabilities. These results suggest that UMC may have a good potential for further clinical application in the future.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available