Study of Albumin Oxidation in COVID-19 Pneumonia Patients: Possible Mechanisms and Consequences

Oxidative stress induced by neutrophils and hypoxia in COVID-19 pneumonia leads to albumin modification. This may result in elevated levels of advanced oxidation protein products (AOPPs) and advanced lipoxidation end-products (ALEs) that trigger oxidative bursts of neutrophils and thus participate in cytokine storms, accelerating endothelial lung cell injury, leading to respiratory distress. In this study, sixty-six hospitalized COVID-19 patients with respiratory symptoms were studied. AOPPs-HSA was produced in vitro by treating human serum albumin (HSA) with chloramine T. The interaction of malondialdehyde with HSA was studied using time-resolved fluorescence spectroscopy. The findings revealed a significantly elevated level of AOPPs in COVID-19 pneumonia patients on admission to the hospital and one week later as long as they were in the acute phase of infection when compared with values recorded for the same patients 6- and 12-months post-infection. Significant negative correlations of albumin and positive correlations of AOPPs with, e.g., procalcitonin, D-dimers, lactate dehydrogenase, aspartate transaminase, and radiological scores of computed tomography (HRCT), were observed. The AOPPs/albumin ratio was found to be strongly correlated with D-dimers. We suggest that oxidized albumin could be involved in COVID-19 pathophysiology. Some possible clinical consequences of the modification of albumin are also discussed.

Automated summary

Neutrophils and hypoxia in COVID-19 pneumonia induce oxidative stress, leading to modification of albumin and triggering oxidative bursts of neutrophils and cytokine storms. This study found significantly elevated levels of advanced oxidation protein products (AOPPs) in COVID-19 pneumonia patients on admission and during the acute phase of infection compared to post-infection values. AOPPs were negatively correlated with albumin and positively correlated with inflammatory markers, radiological scores, and D-dimers. The AOPPs/albumin ratio was strongly correlated with D-dimers. Oxidized albumin may play a role in the pathophysiology of COVID-19.