Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/ijms232012388
Keywords
stem; progenitor retinal cells; ciliary margin; UbC-StarTrack; clonal analysis; chick embryo
Funding
- MCIN/AEI
- Program Enhancing Mobility between Latin American and Caribbean countries and Europe [EMHE-20016]
- Consejo Nacional de Investigaciones Cientificas y Tecnicas [PIP 00441]
- Universidad de Buenos Aires [00526BA, 00769BA]
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Clonal cell analysis reveals the ontogenic potential of retinal stem/progenitor cells and uncovers neural heterogeneity among different cell types and lineages. Using a chick embryo model, this study tracks retinal cell lineages derived from individual progenitors and shows the presence of multipotent and committed progenitor cells in the early stages of development.
Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
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