4.7 Article

Optimization of Prime Editing in Rice, Peanut, Chickpea, and Cowpea Protoplasts by Restoration of GFP Activity

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2

Sudip Biswas et al.

Summary: In this study, the CRISPR-Cas9 genome editing technology was successfully applied to disrupt a major allergen gene in peanut protoplasts. The polyethylene glycol (PEG)-mediated protoplast transformation system proved to be a rapid and effective tool for transient expression analysis and sgRNA validation in peanut.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Review Biochemistry & Molecular Biology

CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review

Adnan Rasheed et al.

Summary: Legumes are vital for food and protein production worldwide. Genome editing, particularly using CRISPR/Cas9, offers a promising approach to improve legume crops. This review provides an overview of recent developments in genome editing of major legumes, highlighting the versatility and potential of CRISPR/Cas9 as a reliable and cost-effective tool.

MOLECULAR BIOLOGY REPORTS (2022)

Article Biotechnology & Applied Microbiology

An engineered prime editor with enhanced editing efficiency in plants

Yuan Zong et al.

Summary: In this study, we have improved the editing efficiency of prime editing by engineering the structure of the prime editor and combining it with specific guide RNAs. The optimized prime editor showed significantly increased editing efficiency in plant cells, and when combined with engineered guide RNAs, it resulted in the generation of herbicide-tolerant rice plants.

NATURE BIOTECHNOLOGY (2022)

Review Biochemistry & Molecular Biology

Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement

Michael J. Thomson et al.

Summary: Advances in molecular technologies have provided more powerful plant breeding approaches, and the establishment of efficient CRISPR-Cas-based gene editing promises to bridge the gap between forward genetics and reverse genetics, and provide a rapid method to functionally validate genes and alleles identified through studies of natural variation.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Review Genetics & Heredity

Gene-Editing Technologies and Applications in Legumes: Progress, Evolution, and Future Prospects

Mehmet Cengiz Baloglu et al.

Summary: Legumes are rich in protein and phytochemicals, and their genetic traits have been improved using genome editing technologies. However, governmental regulatory restrictions present a concern.

FRONTIERS IN GENETICS (2022)

Article Plant Sciences

DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration

Choun-Sea Lin et al.

Summary: A DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol of wild tomato creates stable and inheritable diploid and tetraploid regenerants, which has significant implications for plant breeding and genetic research.

PLANT PHYSIOLOGY (2022)

Article Plant Sciences

A design optimized prime editor with expanded scope and capability in plants

Wen Xu et al.

Summary: This study demonstrated that using N-terminal reverse transcriptase-Cas9 nickase fusion and introducing multiple-nucleotide substitutions in the reverse transcriptase template can significantly enhance the efficiency of prime editing in plants and human cells. The optimized approach achieved higher editing frequencies compared to the original editor, Prime Editor 3, showing the potential to make previously non-editable target sites editable and expand the capabilities of prime editing in the future.

NATURE PLANTS (2022)

Letter Biotechnology & Applied Microbiology

Precise genome modification in tomato using an improved prime editing system

Yuming Lu et al.

PLANT BIOTECHNOLOGY JOURNAL (2021)

Article Biotechnology & Applied Microbiology

Tomato protoplasts as cell target for ribonucleoprotein (RNP)-mediated multiplexed genome editing

Alessandro Nicolia et al.

Summary: This study demonstrates the feasibility of using PEG-mediated RNPs for multiplexing genome editing in tomato protoplasts, showing high efficiency in editing outcomes. This highlights the potential application of RNPs in multiplexing genome editing in plant breeding.

PLANT CELL TISSUE AND ORGAN CULTURE (2021)

Article Engineering, Biomedical

A web tool for the design of prime-editing guide RNAs

Ryan D. Chow et al.

Summary: Prime editing allows diverse genomic alterations to be made at target sites without double-strand breaks or donor templates. However, designing pegRNAs can be complex and time-consuming, as they require customization for each edit. The web tool pegFinder facilitates the rapid design of pegRNAs for downstream experimentation, with the incorporation of sgRNA predictions and nomination of secondary nicking sgRNAs for improved editing efficiency.

NATURE BIOMEDICAL ENGINEERING (2021)

Article Biochemistry & Molecular Biology

First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts

Sapna Badhan et al.

Summary: The current genome editing system CRISPR/Cas9 has been proven effective in plant applications, with the DNA-free editing showing promise in achieving targeted mutagenesis in chickpea. By selecting drought tolerance-related genes 4CL and RVE7 for editing in chickpea protoplast, this study demonstrated high efficiency in editing the RVE7 gene, providing insights into gene functions under drought stress in chickpea.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biotechnology & Applied Microbiology

High-efficiency prime editing with optimized, paired pegRNAs in plants

Qiupeng Lin et al.

Summary: Designing prime binding sites with a certain melting temperature and using two pegRNAs can substantially enhance the efficiency of prime editing.

NATURE BIOTECHNOLOGY (2021)

Review Biotechnology & Applied Microbiology

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Jin-Jun Yue et al.

Summary: Protoplasts play a crucial role in validating gene editing tools and conducting DNA-free gene editing in crops with complex genomes, long juvenile periods, heterosis, and/or self-incompatibility in the CRISPR/Cas system. The applications of protoplast technology in CRISPR/Cas-based breeding have significant implications for crop improvement.

FRONTIERS IN GENOME EDITING (2021)

Review Biochemistry & Molecular Biology

CRISPR ribonucleoprotein-mediated genetic engineering in plants

Yingxiao Zhang et al.

Summary: CRISPR-derived biotechnologies have revolutionized genetic engineering in plants, but still face challenges such as editing efficiency and off-target mutagenesis.

PLANT COMMUNICATIONS (2021)

Review Biotechnology & Applied Microbiology

Base editing in crops: current advances, limitations and future implications

Rukmini Mishra et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Editorial Material Biotechnology & Applied Microbiology

Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing

Kutubuddin A. Molla et al.

TRENDS IN BIOTECHNOLOGY (2020)

Letter Biotechnology & Applied Microbiology

Engineering herbicide resistance via prime editing in rice

Haroon Butt et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Letter Biotechnology & Applied Microbiology

Precision genome engineering in rice using prime editing system

Kai Hua et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Letter Biochemistry & Molecular Biology

Plant Prime Editors Enable Precise Gene Editing in Rice Cells

Xu Tang et al.

MOLECULAR PLANT (2020)

Letter Biochemistry & Molecular Biology

Precise Modifications of Both Exogenous and Endogenous Genes in Rice by Prime Editing

Huiyuan Li et al.

MOLECULAR PLANT (2020)

Letter Biochemistry & Molecular Biology

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

Wen Xu et al.

MOLECULAR PLANT (2020)

Article Biotechnology & Applied Microbiology

Prime genome editing in rice and wheat

Qiupeng Lin et al.

NATURE BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

Targeted, efficient sequence insertion and replacement in rice

Yuming Lu et al.

NATURE BIOTECHNOLOGY (2020)

Article Genetics & Heredity

PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts

Rodrigo Ribeiro Arnt Sant'Ana et al.

GENES (2020)

Article Biotechnology & Applied Microbiology

Efficient genome editing using CRISPR-Cas9 RNP delivery into cabbage protoplasts via electro-transfection

Myoung Hui Lee et al.

PLANT BIOTECHNOLOGY REPORTS (2020)

Article Biotechnology & Applied Microbiology

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Yuan-Yuan Jiang et al.

GENOME BIOLOGY (2020)

Article Biochemistry & Molecular Biology

Development of Plant Prime-Editing Systems for Precise Genome Editing

Rongfang Xu et al.

PLANT COMMUNICATIONS (2020)

Review Biotechnology & Applied Microbiology

CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications

Kutubuddin A. Molla et al.

TRENDS IN BIOTECHNOLOGY (2019)

Article Biotechnology & Applied Microbiology

Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts

Bent Larsen Petersen et al.

BMC BIOTECHNOLOGY (2019)

Article Multidisciplinary Sciences

Search-and-replace genome editing without double-strand breaks or donor DNA

Andrew V. Anzalone et al.

NATURE (2019)

Article Biochemistry & Molecular Biology

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

Florian Veillet et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

Article Biotechnology & Applied Microbiology

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration

Choun-Sea Lin et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

Review Genetics & Heredity

Base editing: precision chemistry on the genome and transcriptome of living cells

Holly A. Rees et al.

NATURE REVIEWS GENETICS (2018)

Article Plant Sciences

Precision genome engineering through adenine base editing in plants

Beum-Chang Kang et al.

NATURE PLANTS (2018)

Article Biotechnology & Applied Microbiology

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Chao Li et al.

GENOME BIOLOGY (2018)

Letter Biochemistry & Molecular Biology

Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System

Yuming Lu et al.

MOLECULAR PLANT (2017)

Letter Biochemistry & Molecular Biology

Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System

Jingying Li et al.

MOLECULAR PLANT (2017)

Article Biotechnology & Applied Microbiology

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

Yuan Zong et al.

NATURE BIOTECHNOLOGY (2017)

Article Biotechnology & Applied Microbiology

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Zenpei Shimatani et al.

NATURE BIOTECHNOLOGY (2017)

Article Biochemistry & Molecular Biology

A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants

Tomas Cermak et al.

PLANT CELL (2017)

Article Multidisciplinary Sciences

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Alexis C. Komor et al.

NATURE (2016)

Article Multidisciplinary Sciences

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

Kabin Xie et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Biochemical Research Methods

Genome editing in rice and wheat using the CRISPR/Cas system

Qiwei Shan et al.

NATURE PROTOCOLS (2014)

Article Biochemical Research Methods

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

Fu-Hui Wu et al.

PLANT METHODS (2009)