Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms23168918
Keywords
umbilical cord; cord lining epithelial cells; skin keratinocytes; cutaneous wounds; wound healing; CLECs
Funding
- National Medical Research Council, Ministry of Health, Singapore [NMRC/CNIG/1106/2013, OFIRG20nov-0024]
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Human umbilical cord lining epithelial cells (CLECs) are a natural and ethical source of cells that can be recovered from routinely discarded cords. Using CLECs to treat cutaneous wounds has been shown to be feasible. Flow cytometry and skin organotypic cultures revealed differences in the expression level and location of basal cell markers between CLECs and skin keratinocytes. In a porcine wound model, CLEC application promoted accelerated healing with no adverse reactions. CLECs have the potential to be a non-native and safe source of cells for treating cutaneous wounds.
Human umbilical cord lining epithelial cells [CLECs) are naive in nature and can be ethically recovered from cords that are routinely discarded. The success of using oral mucosal epithelial cells for cornea defects hints at the feasibility of treating cutaneous wounds using non-native CLECs. Herein, we characterized CLECs using flow cytometry (FC) and skin organotypic cultures in direct comparison with skin keratinocytes (KCs). This was followed by wound healing study to compare the effects of CLEC application and the traditional use of human skin allografts (HSGs) in a porcine wound model. While CLECs were found to express all the epidermal cell markers probed, the major difference between CLECs and KCs lies in the level of expression (in FC analysis) as well as in the location of expression (of the epithelium in organotypic cultures) of some of the basal cell markers probed. On the pig wounds, CLEC application promoted accelerated healing with no adverse reaction compared to HSG use. Though CLECs, like HSGs, elicited high levels of local and systemic immune responses in the animals during the first week, these effects were tapered off more quickly in the CLEC-treated group. Overall, the in vivo porcine data point to the potential of CLECs as a non-native and safe source of cells to treat cutaneous wounds.
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