Increasing the Grain Yield and Grain Protein Content of Common Wheat (Triticum aestivum) by Introducing Missense Mutations in the Q Gene

Grain yield (GY) and grain protein content (GPC) are important traits for wheat breeding and production; however, they are usually negatively correlated. The Q gene is the most important domestication gene in cultivated wheat because it influences many traits, including GY and GPC. Allelic variations in the Q gene may positively affect both GY and GPC. Accordingly, we characterized two new Q alleles (Q(s1) and Q(c1)-N8) obtained through ethyl methanesulfonate-induced mutagenesis. Compared with the wild-type Q allele, Q(s1) contains a missense mutation in the sequence encoding the first AP(2) domain, whereas Q(c1)-N8 has two missense mutations: one in the sequence encoding the second AP(2) domain and the other in the microRNA172-binding site. The Q(s1) allele did not significantly affect GPC or other processing quality parameters, but it adversely affected GY by decreasing the thousand kernel weight and grain number per spike. In contrast, Q(c1)-N8 positively affected GPC and GY by increasing the thousand kernel weight and grain number per spike. Thus, we generated novel germplasm relevant for wheat breeding. A specific molecular marker was developed to facilitate the use of the Q(c1)-N8 allele in breeding. Furthermore, our findings provide useful new information for enhancing cereal crops via non-transgenic approaches.

Automated summary

In this study, two new Q alleles (Q(s1) and Q(c1)-N8) were obtained through ethyl methanesulfonate-induced mutagenesis. The Q(s1) allele did not significantly affect grain protein content (GPC) or other processing quality parameters, but it adversely affected grain yield (GY). In contrast, the Q(c1)-N8 allele positively affected GPC and GY. This research provides novel germplasm for wheat breeding and useful information for enhancing cereal crops.