Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/ijms231810454
Keywords
biocomposites; hydrolytic degradation; sterilization; PLA; hydroxyapatite
Funding
- Foundation for Polish Sciences by the European Union under the European Regional Development Fund [POIR.04.04.00-00-16D7/18]
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Composites based on PLA and HA were prepared using a thermally induced phase separation method. The degradation and pore size distribution of the composites were affected by the preparation, sterilization, and incubation processes, as well as the addition of HA. The inclusion of HA reduced the degradation of PLA and showed no cytotoxicity, indicating its potential for biomedical applications.
Composites based on polylactide (PLA) and hydroxyapatite (HA) were prepared using a thermally induced phase separation method. In the experimental design, the PLA with low weight-average molar mass (M-w) and high M-w were tested with the inclusion of HA synthesized as whiskers or hexagonal rods. In addition, the structure of HA whiskers was doped with Zn, whereas hexagonal rods were mixed with Sr salt. The composites were sterilized and then incubated in phosphate-buffered saline for 12 weeks at 37 degrees C, followed by characterization of pore size distribution, molecular properties, density and mechanical strength. Results showed a substantial reduction of PLA M-w for both polymers due to the preparation of composites, their sterilization and incubation. The distribution of pore size effectively increased after the degradation process, whereas the sterilization, furthermore, had an impact on pore size distribution depending on HA added. The inclusion of HA reduced to some extent the degradation of PLA quantitatively in the weight loss in vitro compared to the control without HA. All produced materials showed no cytotoxicity when validated against L929 mouse skin fibroblasts and hFOB 1.19 human osteoblasts. The lack of cytotoxicity was accompanied by the immunocompatibility with human monocytic cells that were able to detect pyrogenic contaminants.
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