Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/ijms23179575
Keywords
PPARGC1A; miR-193b-3p; mitochondrial biogenesis; muscle metabolism; skeletal muscle development
Funding
- Local Innovative and Research Teams Project of Guangdong Province [2019BT02N630]
- Natural Scientific Foundation of China [U1901206, 31761143014]
- Guangzhou Science and Technology Key Project [202103000084]
- China Agriculture Research System [CARS-41-G03]
- China Postdoctoral Science Foundation [2022M710052]
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This study reveals the role of PPARGC1A in chicken muscle, showing that it affects muscle quality and muscle mass through regulating mitochondrial biogenesis and muscle metabolism. The expression level of PPARGC1A is regulated by miR-193b-3p. These findings contribute to our understanding of the genetic regulation of chicken skeletal muscle development.
Meat production performance is one of the most important factors in determining the economic value of poultry. Myofiber is the basic unit of skeletal muscle, and its physical and chemical properties determine the meat quality of livestock and poultry to a certain extent. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) as a transcriptional coactivator has been found to be widely involved in a series of biological processes. However, PPARGC1A is still poorly understood in chickens. In this manuscript, we reported that PPARGC1A was highly expressed in slow-twitch myofibers. PPARGC1A facilitated mitochondrial biogenesis and regulated skeletal muscle metabolism by mediating the flux of glycolysis and the TCA cycle. Gain- and loss-of-function analyses revealed that PPARGC1A promoted intramuscular fatty acid oxidation, drove the transformation of fast-twitch to slow-twitch myofibers, and increased chicken skeletal muscle mass. Mechanistically, the expression level of PPARGC1A is regulated by miR-193b-3p. Our findings help to understand the genetic regulation of skeletal muscle development and provide a molecular basis for further research on the antagonism of skeletal muscle development and fat deposition in chickens.
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