Journal
CARBOHYDRATE POLYMERS
Volume 122, Issue -, Pages 180-188Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.01.008
Keywords
Alginate; Ratio of mannuronic to guluronic acid (M/G); HPAEC-PAD; Hydrolysis conditions
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Funding
- National Natural Science Foundation of China [81473179]
- Jiangsu Specially-Appointed Professor Research Funding [SR13200113]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [YX13200111]
- Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases [BM2013003]
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Alginate is a linear and acidic polysaccharide, composed of (1 -> 4) linked beta-D-mannuronic acid (ManA) and alpha-L-guluronic acid (GulA). The ratio of ManA to GulA (M/G) is one of the most important factors for the application of alginate and its derivatives in various areas. In this work, a robust and accurate method was developed to analyze M/G using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The impact of hydrolysis conditions on the release patterns of ManA and GulA from alginate and its derivatives was investigated. The release patterns of ManA and GulA need to be considered separately to obtain an accurate M/G. Several hydrolysis conditions were established that released ManA and GulA completely and maintained these saccharide residues intact. The proper M/G of alginates from different sources and its derivatives could then be calculated by integration of the corresponding ManA and GulA peaks. (C) 2015 Elsevier Ltd. All rights reserved.
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