Journal
CARBOHYDRATE POLYMERS
Volume 130, Issue -, Pages 280-289Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.05.008
Keywords
Nanoparticles; Chitosan derivatives; Newcastle disease; Mucosal immune delivery system; Sustained release
Categories
Funding
- Key Program of Natural Science Foundation of Heilongjiang Province of China [ZD2015002]
- Innovative Research Team for Agricultural Microbiology Fermentation Technology at Heilongjiang Provincial University [2012td009]
- Changjiang Scholar Candidates Program for Provincial Universities in Heilongjiang [2014CJHB005]
- Scientific and Technological Key Project of Heilongjiang Province [GC13B403]
- Early Research and Development Cultivation Project of Scientific and Technological Achievements Industrialization for Provincial Universities in Heilongjiang [1253CGZH10]
- Technological Innovation Talent Foundation of Harbin [2013RFQXJ030]
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A novel complex chitosan derivative, O-2'-hydroxypropyltrimethyl ammonium chloride chitosan (O-2'-HACC), was synthesized and used to make nanoparticles as a delivery vehicle for live attenuated Newcastle disease vaccine. We found that O-2'-HACC had high antimicrobial activity, low toxicity, and a high safety level. Newcastle disease virus (NDV) was then encapsulated in the O-2'-HACC nanoparticles (NDV/La Sota-O-2'-HACC-NPs) by the ionic crosslinking method, and the properties of the resulting nanoparticles were determined by transmission electron microscopy, Zeta potential analysis, Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and X-ray diffraction. NDV/La Sota-O-2'-HACC-NPs had regular spherical morphologies and high stability, with an encapsulation efficiency of 95.68 +/- 2.2% and a loading capacity of 58.75 +/- 4.03%. An in vitro release assay indicated that release of NDV from NDV/La Sota-O-2'-HACC-NPs occurred slowly. Specific pathogen-free chickens immunized with NDV/La Sota-O-2'-HACC-NPs intranasally had much stronger cellular, humoral and mucosal immune responses than did those immunized intramuscularly or with live attenuated Newcastle disease vaccine. NDV/La Sota-O-2'-HACC-NPs are a novel drug delivery carrier with immense potential in medical applications. (C) 2015 Elsevier Ltd. All rights reserved.
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