Journal
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
Volume 33, Issue -, Pages 28-32Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2015.10.002
Keywords
Cytochrome P450 monooxygenases; Eosin Y; Poly(3-hydroxybutyrate); Solar-tracking system
Funding
- National Research Foundation (NRF) via the National Leading Research Laboratory [NRF-2013R1A2A1A05005468]
- Intelligent Synthetic Biology Center of Global Frontier R&D Project, Republic of Korea [2011-0031957, NRF-2014M3A6A8066443]
- KAIST High Risk High Return Project (HRHRP)
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Cytochrome P450 monooxygenases which catalyze a remarkable variety of oxidative transformation are of exceptional interest for the synthesis of fine chemicals. However, due to the instability and requirement of expensive cofactor, P450s have yet to be extensively used for industry. Here, we developed a new platform that combines a solar-driven cofactor regeneration and immobilization of P450 on biopolymer. Through photochemical cofactor regeneration using eosin Y as a photosensitizer, P450-P(3HB) complex successfully catalyzed a O-dealkylation reaction. Furthermore, using the P450-P(3HB) complex and a solar-tracking module, we achieved a P450-catalyzed reaction under natural sunlight for four consecutive days in a preparative scale. (C) 2015 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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