4.7 Article

Antioxidant and anti-inflammatory properties of an aminoglycan-rich exopolysaccharide from the submerged fermentation of Bacillus thuringiensis

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 220, Issue -, Pages 1010-1020

Publisher

ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.08.116

Keywords

Exopolysaccharides; Bacillus thuringiensis IX-01; High-cell-density fermentation; Antioxidant; Anti-inflammation

Funding

  1. National Key Research and Development Program of China [2021YFC2101100, 2018YFC1604105-5]
  2. Program of Introducing Talents of Discipline [111-2-06]

Ask authors/readers for more resources

This study extracted the exopolysaccharide BPS-2 from Bacillus thuringiensis and found that it has strong anti-digestive capacity, as well as anti-inflammatory and antioxidant effects in vitro. These findings provide a basis for biopharmaceutical applications of BPS-2.
Proteins from Bacillus thuringiensis are widely used as biopesticides but little is known about its exopoly-saccharides. The exopolysaccharide BPS-2 was extracted from B. thuringiensis IX-01 after high-cell-density fermentation. BPS-2 is a heteropolysaccharide (molecular weight 29.36 kDa) composed of D-galactosamine, arabinose, glucosamine, glucose, and mannose in molar ratios 5.53: 1.77:4.74:3.24:1. In vitro upper gastrointestinal simulations showed that BPS-2 has strong anti-digestive capacity, with scavenging of DPPH, hydroxyl, ABTS, and superoxide anions radicals of 31.34 +/- 1.67 %, 32.43 +/- 3.01 %, 34.31 +/- 2.12 %, and 48.53 +/- 3.55 %, respectively, after BPS-2 entered the colon. It significantly inhibited production of lipopolysaccharide-induced nitric oxide and multiple pro-inflammatory cytokines and had proliferative effects on RAW 264.7 cells. BPS-2 inhibited malondialdehyde secretion and elevated activities of glutathione peroxidase, superoxide dismutase, and total antioxidants, significantly improving the antioxidant status of inflammation model cells. This first report of the in vitro anti-inflammation and antioxidant properties of BPS-2 from B. thuringiensis provides a basis for biopharmaceutical applications.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available