Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 222, Issue -, Pages 2368-2374Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.10.023
Keywords
Enzyme stabilization; Lipase lid opening; Nanoscale enzyme reactors; Lipase -catalyzed transesterification; Mesoporous silica
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2020R1A2C3009649]
- Ministry of Environment
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In this study, a nanoscale enzyme reactor (NER) for lipase from Rhizopus oryzae was prepared, which showed high stability and activity. The NER demonstrated great potential as an environmentally-friendly nanobiocatalyst for various lipase applications, including biodiesel production.
Lipases catalyze a wide range of industrially important reactions, including the transesterification of triglycerides with alcohols for biodiesel production, and the stabilization of lipases are critical to achieve their recycled uses. Here, nanoscale enzyme reactor (NER) of lipase from Rhizopus oryzae (LP) was prepared via a simple two-step process, comprising of enzyme adsorption into magnetically-separable mesoporous silica and follow-up crosslinking of adsorbed enzymes. In aqueous phase, the specific hydrolysis activity of NER-LP was 4.7 times lower than that of free LP. On the other hand, however, the specific transesterification activity of NER-LP (130.4 mu mol/ min/mg LP) in organic phase for biodiesel production was 50 times higher than that of free LP (2.6 mu mol/min/ mg LP). These results reveal that the enzyme crosslinking for the preparation of NER does not interfere with the interfacial activation of LP molecules, opening the lid of LP active site under an optimal hydrophobic environment provided by the combination of organic solvent and mesoporous silica. Magnetic separation and optimized washing protocol facilitated the recycled uses of NER-LP. Highly stable and active NER-LP in magneticallyseparable mesoporous silica has demonstrated its great potentials as an environmentally-friendly nanobiocatalyst for various lipase applications, including plasticizers, biosurfactants, functional fatty acids, as well as recyclable biodiesel production.
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