Journal
JOURNAL OF IMMUNOLOGY
Volume 198, Issue 2, Pages 657-668Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1601301
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Funding
- Rheumatology Research Foundation Scientist Development Award
- Rosalind Russell Medical Research Foundation Bechtel Award
- University of California San Francisco-Stanford Arthritis Center of Excellence
- Northern California Arthritis Foundation
- National Institute of Allergy and Infectious Disease of the National Institutes of Health [P01 AI091580]
- Rheumatology Research Foundation Within Our Reach grant
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Distinguishing true Ag-stimulated lymphocytes from bystanders activated by the inflammatory milieu has been difficult. Nur77 is an immediate early gene whose expression is rapidly upregulated by TCR signaling in murine T cells and human thymocytes. Nur77-GFP transgenes serve as specific TCR and BCR signaling reporters in murine transgenic models. In this study, we demonstrate that endogenous Nur77 protein expression can serve as a reporter of TCR and BCR specific signaling in human PBMCs. Nur77 protein amounts were assessed by immunofluorescence and flow cytometry in T and B cells isolated from human PBMCs obtained from healthy donors that had been stimulated by their respective Ag receptors. We demonstrate that endogenous Nur77 is a more specific reporter of Ag-specific signaling events than the commonly used CD69 activation marker in both human T and B cells. This is reflective of the disparity in signaling pathways that regulate the expression of Nur77 and CD69. Assessing endogenous Nur77 protein expression has great potential to identify Ag-activated lymphocytes in human disease.
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