Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 183, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.indcrop.2022.114905
Keywords
HPLC; Carbopol-940; Ethosomal gel (EG); Non-ethosomal gel (NEG); in-vitro; Permeation
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This study aimed to develop and evaluate a plant extract-loaded ethanol gel for potential cosmeceutical applications. The results showed that the plant extract exhibited antioxidant activity against oxidative stress. The optimized ethanol gel showed good stability and release properties.
The study was aimed to develop and characterize plant extract (Brassica oleraceae) loaded ethosomal gel for the valuation of cosmeceutical applications with a credence that botanical extracts have a greater capability to be employed in the formulation of advanced pharmaceuticals. Phytochemical profiling via FT-IR analysis, total bioactive contents of different polarity extracts (ethanol, dichloromethane, ethyl-acetate and chloroform) and quantification by using High Performance Liquid Chromatographic (HPLC) assay was carried out. Capabilities against oxidative stress were prodded through 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), reduction potential analyses. Besides this, a correlation amongst total bioactive contents (TPC/TFC) and antioxidant potentials (biological activities) was studied by multi-variate statistical analysis. An augmented ethosomal preparation, after characterization and stability studies was incorporated to the gel matrix of Carbopol-940; Test/ EG (Ethosomal gel) with a Control/NEG (Non-ethosomal gel) formulation. Both formulations were then subjected to in-vitro characterization (organoleptic evaluation, pH, conductivity and rheology) of 90 days. By permeation studies flux (mu g/cm2/hr); enhancement ratio was calculated and characterization of release data was done by using different kinetic models. The plant extracts revealed the presence of different functional groups along with 39.6 +/- 0.5 mgGAE/g; 26.56 +/- 0.4 mgQE/g phenolic and flavonoid contents, respectively; HPLC assay confirmed the existence of Quercetin, Gallic, Caffiec, Benzoic, Syringic and Sinapic Acids with highest antioxidant activity of 87.75% +/- 1.18 in hydro-alcoholic fraction. Optimized ethosomal formulation exhibited entrapment efficiency of 68.57 +/- 1.25, smallest vesicle size of 228.6 +/- 1.17 nm with - 21.3 +/- 0.15, zeta potential and a homogeneous size distribution of 0.233 +/- 0.02. Significant results were obtained after in-vitro characterization. Both formulations followed Korsmeyer-peppas model with flux 35.499 and 21.731 mu g/cm2/hr respectively and enhancement ratio 1.63. Thus, a stable ethosomal loaded gel of Brassica oleraceae extract was successfully developed and characterized for cosmeceutical potential.
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