Journal
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
Volume 58, Issue 6, Pages 903-910Publisher
SPRINGER
DOI: 10.1007/s11627-022-10304-6
Keywords
2,4-Dichlorophenoxyacetic acid; Thidiazuron; 6-Benzylaminopurine; 1-Naphthaleneacetic acid; Indirect organogenesis; Chayote
Categories
Funding
- Consejo Nacional de Ciencia y Tecnologia (National Council of Science and Technology) [932915]
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This study reported an efficient protocol for the indirect organogenesis of Sechium edule, a Mesoamerican species, utilizing different hormones to successfully induce callus formation and shoot regeneration, providing important insights for the propagation of Sechium edule.
This article reports an efficient protocol for the indirect organogenesis of Sechium edule, a Mesoamerican species with important nutritional and pharmaceutical characteristics. The in vitro establishment was carried out with hypocotyl segments; subsequently, the formation of calluses was induced from leaves and nodal stem segments using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5, 1.0, and 1.5 mg L-1). Then, shoot formation was induced using different concentrations of thidiazuron (TDZ; 0.5, 1.0, and 1.5 mg L-1) in medium supplemented with 1.0 mg L -1 benzylaminopurine (BAP). Different concentrations of 1-naphthaleneacetic acid (NAA; 1.0, 2.0, and 3.0 mg L-1) were assessed to stimulate shoot rooting; chlorophyll-a, chlorophyll-b, and total chlorophyll were quantified during this stage. The results showed that callus formation and weight increased in parallel with 2,4-D concentration, with nodal segments responding favorably. Shoot regeneration was higher in medium supplemented with 0.5 mg L-1 TDZ plus 1.0 mg L-1 BAP. Shoot elongation and rooting were greater using 1.0 mg L-1 NAA, while chlorophyll-a, chlorophyll-b, and total chlorophyll contents were higher in medium without NAA. A 90% plant survival was recorded after acclimatization.
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