Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 438, Issue -, Pages 21-25Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2016.08.008
Keywords
Periodontal disease; Gingival crevicular fluid; Ligature-induced periodontal disease; Mice
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Funding
- National Institute of Dental and Craniofacial Research (NIDCR) [DE-03420, DE-18499, DE-19917, T32 DE 7327-12]
- Brain Circulation Program to Develop New Leaders for International Dental Education Course through International Collaborative Dental Research
- King Abdulaziz University of Saudi Arabia
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Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10 min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-lb, TNF-alpha, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD. (C) 2016 Published by Elsevier B.V.
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