Journal
CARBOHYDRATE POLYMERS
Volume 133, Issue -, Pages 154-162Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.07.018
Keywords
Rhizoma Acori Tatarinowii; Polysaccharides; Antioxidative activity; Immunological activity
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Funding
- Program for New Century Excellent Talents in University [NCET-13-0480]
- National Natural Science Foundation of China (NSFC) [31270860]
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Rhizoma Acori Tatarinowii is widely used in traditional Chinese medicine. In this study, three novel polysaccharides designated RATPW, RATPS1 and RATPS2 were isolated from Rhizoma Acori Tatarinowii by DEAE-52 cellulose chromatography. Their structures were characterized using physicochemical and spectral methods. Chemical analysis indicated that RATPW (6.5 x 10(3) Da) mainly composed of glucose and fructose. RATPS1 (1.5 x 10(5) Da) contained galactose and arabinose, while RATPS2 (5.3 x 10(4) Da) contained similar to 49.5% galacturonic acid along with rhamnose, fructose, galactose, and arabinose. In vitro, RATPS2 showed the most significant scavenging activity on DPPH and hydroxyl radical. Three polysaccharides could protect the PC12 cells from H2O2-induced damage. Immunological tests indicated that both RATPW and RATPS2 significantly stimulated NO production and phagocytic activity in RAW264.7, and promoted splenocyte proliferation. These data suggested that polysaccharides RATPW and RATPS2 had the potential as novel natural sources of antioxidative and immunopotentiating agents. (C) 2015 Elsevier Ltd. All rights reserved.
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