Journal
GENOMICS
Volume 114, Issue 5, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2022.110441
Keywords
Chloridea (Heliothis) subflexa; Genome assembly; Reference-assisted assembly approach; Heliothinae
Funding
- North Carolina State University W. M. Keck Center for Behavioral Biology
- USDA NIFA Biotechnology Risk Assessment [2016-33522-25640, 2019-33522-29992]
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This paper reports the genome assembly of Chloridea subflexa and Chloridea virescens, two closely related noctuid species, and examines the feasibility of reference-assisted assembly. The results show that reference-assisted assembly has the potential to improve the contiguity and completeness of insect genomic resources with low additional costs.
Chloridea subflexa and Chloridea virescens are a pair of closely related noctuid species exhibiting pheromone-based sexual isolation and divergent host plant preferences. We produced a novel Illumina short read C. subflexa genome assembly and an improved C. virescens genome assembly, which offer opportunities to study the genomic basis for evolutionarily important traits in this lepidopteran family with few genomic resources. We then examined the feasibility of reference-assisted assembly, an approach that leverages existing high quality genomic resources for genome improvement in closely related taxa and applied it to our Heliothine genomes. Our work demonstrates that reference-assisted assembly has the potential to enhance contiguity and completeness of existing insect genomic resources with minimal additional laboratory costs. We conclude by discussing both the potential and pitfalls of reference-assisted assembly according to the intended downstream assembly application.
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