Journal
FOOD CHEMISTRY
Volume 390, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133204
Keywords
Medical nutrition therapy; Food quality control; Matrix effect; Chromatography; Measurement uncertainty
Ask authors/readers for more resources
This research developed a method based on high-performance liquid chromatography and diode array detection to determine free amino acids in amino acid-based infant formulas and amino acid-modified medical foods. The method showed good linearity and repeatability, making it suitable for analyzing free amino acids in seven powdered medical foods.
This research aims at determining some free amino acids in amino acid-based infant formulas and amino acidmodified medical foods for inborn errors of metabolism to prove their quality. A method based on highperformance liquid chromatography and diode array detection was developed and validated. Then, overall uncertainty was estimated by the bottom-up approach. Applying the weighted least squares regression method suggested good linearity with coefficient of determinations >= 0.9960. The limits of detection were calculated between 0.01 and 0.28 mu g/mL. The most repetitive recovery values were obtained in the range of 91-108%, with RSDs <= 15%. The expanded uncertainties were below 20% for most amino acids. The contributions of linear regression and repeatability are two main factors in estimating overall uncertainty. The results offer this method as a simple and easy procedure for analyzing free amino acids in seven powdered medical foods designed for phenylketonuria, maple syrup urine disease, methylmalonic, and propionic acidemia.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available