4.7 Article

Modulation of oxidative stress and gut microbiota by selenium-containing peptides from Cardamine enshiensis and structural-based characterization

Journal

FOOD CHEMISTRY
Volume 395, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133547

Keywords

Se-containing peptides; Antioxidant activity; Gut microbiome; Structures; Molecular docking

Funding

  1. China Postdoctoral Sci-ence Foundation [2020M672312]

Ask authors/readers for more resources

This study investigated the antioxidant properties of Se-containing peptides from Cardamine enshiensis (SeCPPs) and their impact on gut microbiota. The results showed that SeCPPs had a significant antioxidant effect and could modulate the gut microbiome in mice. In addition, SeCPPs improved memory in aging mice and reduced Proteobacteria and Akkermansia abundances in antibiotic-treated mice.
The antioxidant properties of Se-containing peptides from Cardamine enshiensis (SeCPPs) and their impact on gut microbiota were studied in D-galactose (D -gal)-injected mice and antibiotic-treated mice. The structures of SeCPPs were identified by UPLC-Q-Extractive Orbitrap MS. In D-gal ageing mice, SeCPPs were associated with significantly decreased acetyl cholinesterase (AchE) activity, malondialdehyde (MDA) content, increased glutathione peroxidase (GSH-Px) activity, downregulated tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) levels (p < 0.05), and improved memory. In antibiotic-treated mice, SeCPPs were associated with reduced Proteobacteria and evaluated Akkermansia abundances (p < 0.01). Eighty-five Se -containing peptides were identified in SeCPPs. Peptides such as RV-SeM-I, RA-SeM-T and R-SeC-K showed low binding energy with 1,1-diphenyl-2-picrylhydrazyl (DPPH.), and their binding affinities were confirmed by molecular docking. Overall, compared with Na2SeO3 and SeMet, SeCPPs showed superior antioxidant activity via their association with higher antioxidant enzyme activity, scavenging free radical properties and gut microbiome modulation.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available