Journal
FOOD CHEMISTRY
Volume 390, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133207
Keywords
Piceid; Tyrosinase; Anti-browning; Molecular docking
Funding
- National key R&D Program of China [2018YFC1602206]
- National Natural Science Foundation of China [21878107, 21978101]
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Different mechanisms can be used to inhibit tyrosinase and prevent quality losses caused by enzymatic browning in fruits and vegetables. This study found that PI/PIA can inhibit the oxidation of L-DOPA by tyrosinase, leading to a conformational change in the enzyme's secondary structure. Further analysis revealed the mechanism of how PI/PIA affects tyrosinase without pre-incubation. The anti-browning effects of PI/PIA were also demonstrated in potato models.
Different mechanisms for inhibiting tyrosinase can be exploited to avoid quality losses caused by the enzymatic browning of fruits and vegetables. Piceid (PI) and piceid 6 ''-O- azelaic acid ester (PIA) are oxidized by tyrosinase; however, their oxidation products may have inhibitory effects on tyrosinase. This notion is because L-DOPA oxidation was inhibited after the pre-incubation of PI/PIA with tyrosinase, however, L-DOPA oxidation was not affected if this pre-incubation was not performed. Circular dichroism analysis indicated a conformational change in the secondary structure of tyrosinase after pre-incubation. Further, molecular docking and enzyme reaction kinetics assays were employed to reveal the mechanism underlying the effects of PI/PIA on tyrosinase in the absence of pre-incubation with tyrosinase. PI/PIA had anti-browning effects in the potato models. The increased rate of A420 in PI/PIA groups at 24 h were 281% and 279%, which were approximately 2.4- and 2.5-fold lower than that of control (668%).
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