Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 130, Issue -, Pages 472-478Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.09.057
Keywords
Grx 5; Oxidative stress; Innate immunity; Detoxification
Funding
- Key-Area Research and Development Program of Guangdong Province [2021B0202040001]
- earmarked CARS-48
- Central Public-interest Scientific Institution Basal Research Fund
- South China Sea Fisheries Research Institute, CAFS [2021SD15]
- Guangzhou Municipal Science and Technology Project [202206010138]
Ask authors/readers for more resources
In this study, a novel full-length of Glutaredoxin 5 (Sp-Grx 5) was cloned and characterized in the mud crab. Sp-Grx 5 was widely expressed in various tissues with high-level expression in hepatopancreas. It was found to be involved in innate immunity and detoxification, playing important roles in redox homeostasis and innate immune response in crustaceans.
Glutaredoxin (Grx) is a class molecule oxidoreductase, which plays a key role in maintaining redox homeostasis and regulating cell survival pathways. However, the expression pattern and function of Grx remain unknown in the mud crab (Scylla paramamosain). In the present study, a novel full-length of Grx 5 from the mud crab (designated as Sp-Grx 5) was cloned and characterized. The open reading frame of Sp-Grx 5 was 441 bp, which encoded a putative protein of 146 amino acids. The amino acid sequence of Sp-Grx 5 contained a typical C-G-F-S redox active motif and several GSH binding sites. Sp-Grx 5 widely existed in all tested tissues with a high-level expression in hepatopancreas. Subcellular localization showed that Sp-Grx 5 was located in the cytoplasm and nucleus. The expression of Sp-Grx 5 was significantly up-regulated after Vibrio parahaemolyticus infection and cadmium exposure, suggesting that Sp-Grx 5 was involved in innate immunity and detoxification. Furthermore, overexpression of Sp-Grx 5 could improve cells viability after H2O2 exposure. All these results indicated that SpGrx 5 played important roles in the redox homeostasis and innate immune response in crustaceans.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available