Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio-sensitivity and dose-rate findings contributing

In order to achieve the goal of rapid response, effective controland protection of life inlarge-scale radiation events, the IAEA Manual 2011 has been revised based on the data of radio-sensitivity, dose-rate findings. Analyze individual differences in radiation sensitivity using Co-60 radiation (0.27 Gy/min). Chromosomal aberrations with different irradiation dose rates were used to establish the biological dose curve and analyze the excess of the dicentric + ring caused by the dose rate at each dose point; DAPI-images and Metafer 4 were used to capture metaphase images and make further analysis. The data were collected in 2020, Dicentric + ring/100 Cells was 17.5-43.8, the average value was28.32 +/- 6.98. The mean value of Dicentric + ring/100 Cells was 31.37 in males while 25.27 in females, there are significant differences (p < .01). The irradiation dose is dominant, At each dose point, the value of(dicentric chromosome + centric rings)/cell is proportional to dose rate, that is, Y = kx + b, within the dose range of 1-5 Gy, (dicentric chromosome + centric rings)/Cell holds a quadratic linear relationship with dose rate, that is, y = ax(2) + bx + c; The DAPI-images might give you more hints than those of conventional Giemsa-stain. The authors recommend that the IAEA Manual 2011 could be revised based on data of radio-sensitivity and dose-rate, which may contribute to the establishment of a unified dose-response calibration curve and stimulation of potential for automation in cytogenetic biodosimetry. (1) Individual differences of radiosensitivity are very large. (2) At each dose point, (dicentric chromosome + centric rings)/cell is proportional to dose rate, that is, Y = kx + b. (3) (dicentric chromosome + centric rings)/Cell is a quadratic linear relationship with dose rate, that is, y = ax(2) + bx + c. (4) We created a Unity Standard Curve of Biological Dose Estimation. Creating a Unity Standard Curve of Biological Dose, under these circumstances, we can form a joint and rapid response to a nuclear and radiological accident.

Automated summary

In order to achieve rapid response, effective control, and protection of life in large-scale radiation events, the IAEA Manual 2011 has been revised based on data of radio-sensitivity and dose-rate. The authors conducted experiments and found significant individual differences in radio-sensitivity. They also established a quadratic linear relationship between (dicentric chromosome + centric rings)/cell and dose rate. They recommend creating a Unity Standard Curve of Biological Dose for a joint and rapid response to nuclear and radiological accidents.