ex vivo-in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping

When it comes to skin penetration analysis of a topically applied formulation, the number of suitable methods is limited, and they often lack in spatial resolution. In vivo studies are pivotal, especially in the approval of a new product, but high costs and ethical difficulties are limiting factors. For that reason, good ex vivo models for testing skin penetration are crucial. In this study, caffeine was used as a hydrophilic model drug, applied as a 2% (w/w) hydrogel, to compare different techniques for skin penetration analysis. Confocal Raman microspectroscopy (CRM) and tape stripping with subsequent HPLC analysis were used to quantify caffeine. Experiments were performed ex vivo and in vivo. Furthermore, the effect of 5% (w/w) 1,2-pentanediol on caffeine skin penetration was tested, to compare those methods regarding their effectiveness in detecting differences between both formulations.

Automated summary

There is a limited number of suitable methods for skin penetration analysis of topically applied formulations, and they often lack spatial resolution. Good ex vivo models are crucial for testing skin penetration. This study compared different techniques for skin penetration analysis using caffeine as a hydrophilic model drug and tested the effect of 1,2-pentanediol on caffeine skin penetration.