Improving the removal efficiency of nitrogen and organics in vertical-flow constructed wetlands: the correlation of substrate, aeration and microbial activity

Four vertical-flow CWs (VFCWs) with different substrates and aeration conditions were studied on nutrient-removal capacity from synthetic wastewater. Zeolite substrate VFCWs (none-aerated: VFCW-1, aerated: VFCW-3) paralleled with ceramsite (none-aerated:VFCW-2, aerated: VFCW-4) were used to study the removal efficiencies of N and organics, the bacterial community, and the related functional genes. The results indicated that the pollutant removal efficiency was significantly enhanced by intermittent aeration. VFCW-4 (ceramsite with aeration) demonstrated a significant potential to remove NH4+-N (89%), NO3--N (78%), TN (71%), and COD (65%). VFCW-3 and VFCW-4 had high abundances of Amx, amoA, and nirK genes, which was related to NH4+-N and NO2--N removal. The microbial diversity and structure varied with aeration and substrate conditions. Proteobacteria, Actinobacteria, Candidatus, and Acidobacteria were the main bacteria phyla, with the average proportion of 38%, 21%, 19%, and 7% in the VFCWs. Intermittent aeration increased the abundance of Acidobacteria, which was conducive to the removal of organic matters. Overall, ceramsite substrate combined with intermittent aeration has a great potential in removing pollutants in VFCWs.

Automated summary

This study investigated the nutrient-removal capacity of four vertical-flow constructed wetlands (VFCWs) with different substrates and aeration conditions for synthetic wastewater. The results showed that intermittent aeration significantly enhanced pollutant removal efficiency. VFCW-4 with ceramsite substrate and aeration demonstrated great potential in removing NH4+-N, NO3--N, TN, and COD. The microbial diversity and structure varied with aeration and substrate conditions, and ceramsite substrate combined with intermittent aeration increased the abundance of Acidobacteria, which was conducive to the removal of organic matters.