Method of manufacturing and staining microplastics for using in the biological experiments

Nowadays, plastic pollution attracts much attention both from society and scientists. The plastic pollution impact on the environment and human health requires assessment urgently, especially through experimental studies. However, such studies are still scarce because of the lack of standard methods for assessing their effects on living organisms. We have developed a process for manufacturing and staining PVC microparticles for using them in biological and ecological experiments. The electrospinning method has been used to manufacture PVC particles; their morphology and size have been analyzed. The obtained PVC particles are of narrow size range averaging 2-4 mu m in diameter. They are successfully stained with the fluorescent dye Rhodamine B, which stands for the experiments performed in the seawater.

Automated summary

Plastic pollution has garnered much attention, but its impact on the environment and human health requires urgent assessment through experimental studies. However, limited studies exist due to the lack of standardized methods for evaluating their effects on living organisms. This article presents a process for manufacturing and staining PVC microparticles for use in biological and ecological experiments.