☆ 4.8 Article

Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022)

Related references

Note: Only part of the references are listed.

Article Chemistry, Analytical

Universal Exponential Amplification Confers Multilocus Detection of Mutation-Prone Virus

Yue Zhao et al.

Summary: This study proposes a multilocus detection method for SARS-CoV-2 based on improved loop-mediated isothermal amplification and universal primers. The method is able to accurately and rapidly diagnose the virus, and has the capability to identify multiple gene loci. Experimental results demonstrate the significant potential of this method for the diagnosis of SARS-CoV-2 and other infectious diseases.

ANALYTICAL CHEMISTRY (2022)

Add to Collection

Article Engineering, Environmental

Membrane-Based In-Gel Loop-Mediated Isothermal Amplification (mgLAMP) System for SARS-CoV-2 Quantification in Environmental Waters

Yanzhe Zhu et al.

Summary: This study presents a novel mgLAMP system for rapid quantification of SARS-CoV-2 particles in environmental waters. The system can detect virus concentrations as low as 0.96 copies/mL in less than 1 hour. Additionally, the integration of a 3D-printed portable device and smartphone-based imaging with machine learning-based image processing shows promise for large-scale environmental surveillance of SARS-CoV2.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022)

Add to Collection

Article Engineering, Environmental

Respiratory Syncytial Virus (RSV) RNA in Wastewater Settled Solids Reflects RSV Clinical Positivity Rates

Bridgette Hughes et al.

Summary: Wastewater-based epidemiology (WBE) can be used to infer infection trends by measuring the concentration of infectious substances in wastewater. This study found that respiratory syncytial virus (RSV) could be detected in wastewater, and its concentration was closely associated with clinical positivity rates. Since RSV infections have similar clinical presentations to COVID-19 and can be life-threatening, WBE could serve as an important tool to enhance RSV surveillance and public health response efforts.

ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS (2022)

Add to Collection

Article Chemistry, Physical

Sensitively detecting antigen of SARS-CoV-2 by NIR-II fluorescent nanoparticles

Ruibin Hu et al.

Summary: In this study, we developed a lateral flow assay based on NIR-II fluorescence for sensitive detection of SARS-CoV-2 antigen. Compared to traditional methods, the NIR-II LFA has a higher signal-to-background ratio and can successfully detect clinical samples with low antigen concentrations.

NANO RESEARCH (2022)

Add to Collection

Article Chemistry, Analytical

Universally Stable and Precise CRISPR-LAMP Detection Platform for Precise Multiple Respiratory Tract Virus Diagnosis Including Mutant SARS-CoV-2 Spike N501Y

Tong Zhang et al.

Summary: A new CRISPR-LAMP technology has been developed for rapid and accurate diagnosis of respiratory tract viruses, with increased sensitivity and reduced aerosol contamination. The technology combines different techniques and designs to precisely diagnose pathogens and efficiently detect point mutations.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Biophysics

opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection

Rui Wang et al.

Summary: The opvCRISPR system integrates RT-LAMP and Cas12a cleavage to provide a rapid and sensitive detection method for SARS-CoV-2. Its ability to detect at nearly single molecule level in 45 minutes and show 100% agreement with traditional methods when validated with clinical samples make it a promising tool for point-of-care molecular diagnostics in the future.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Biophysics

Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platform

Nathaniel C. Cady et al.

Summary: The study utilized a GC-FP biosensor platform to rapidly and accurately measure COVID-19 antibodies, including multiple antibody isotypes and sample matrices, and developed a machine learning approach to accurately score prior COVID-19 infection based on antibody binding data for all three COVID-19 antigens used in the test.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Editorial Material Engineering, Environmental

Evidence of Foodborne Transmission of the Coronavirus (COVID-19) through the Animal Products Food Supply Chain

Ligang Hu et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2021)

Add to Collection

Article Engineering, Environmental

Occurrence and Distribution of Disinfection Byproducts in Domestic Wastewater Effluent, Tap Water, and Surface Water during the SARS-CoV-2 Pandemic in China

Zhigang Li et al.

Summary: The study investigated the occurrence and distribution of disinfection byproducts (DBPs) in domestic wastewater effluent, tap water, and surface water in China. Results showed that DBP concentrations were higher in wastewater effluents than tap water and surface water, with haloacetic acids (HAAs) and trihalomethanes (THMs) being the most dominant categories. Additionally, the study revealed that HAAs in wastewater effluents had higher risk quotient values for green algae.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2021)

Add to Collection

Article Multidisciplinary Sciences

Comparative analysis of loop-mediated isothermal amplification (LAMP)-based assays for rapid detection of SARS-CoV-2 genes

Daniel Urrutia-Cabrera et al.

Summary: This study compares the performance of three LAMP-based assays targeting different regions of SARS-CoV-2, showing that all three assays can accurately detect 1000 RNA copies in 30 minutes, with the Gene N assay exhibiting no false positives. This highlights the significance of LAMP as a rapid, highly sensitive detection method for SARS-CoV-2 diagnostics.

SCIENTIFIC REPORTS (2021)

Add to Collection

Editorial Material Engineering, Environmental

Campus Collaborations As a Model for Transforming SARS-CoV-2 Wastewater Surveillance Research into Public Health Action

Katrina Smith Korfmacher et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2021)

Add to Collection

Review Engineering, Environmental

Occurrence of various viruses and recent evidence of SARS-CoV-2 in wastewater systems

Waqar Ali et al.

Summary: Viruses are widespread and persistent in wastewater, posing a risk to human health. This review summarizes methods for virus analysis, spatial distribution, and temporal patterns of various viruses in wastewater systems, as well as recent studies on SARS-CoV-2 detection in wastewater. The utility of wastewater-based epidemiology (WBE) for estimating virus occurrence, distribution, genetic diversity, and human health risk assessment is discussed, highlighting the potential use of WBE as an early warning system for public health assessment.

JOURNAL OF HAZARDOUS MATERIALS (2021)

Add to Collection

Article Engineering, Environmental

Wastewater surveillance of SARS-CoV-2 across 40 US states from February to June 2020

Fuqing Wu et al.

Summary: Wastewater-based disease surveillance is a promising approach for monitoring community outbreaks. A nationwide campaign in the US found that SARS-CoV-2 RNA was detected in wastewater samples, with viral concentrations declining from April to May. The detection rate of SARS-CoV-2 in wastewater was positively associated with the size of wastewater treatment plant catchment areas.

WATER RESEARCH (2021)

Add to Collection

Review Public, Environmental & Occupational Health

Point-of-Care Diagnostic Tools for Surveillance of SARS-CoV-2 Infections

Dhanasekaran Sakthivel et al.

Summary: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a highly contagious virus causing COVID-19, with over 212 million confirmed cases and nearly 4.4 million deaths globally as of August 24, 2021. Early diagnosis and isolation are crucial in controlling the spread of SARS-CoV-2, with standard RT-PCR protocols being time-consuming and expensive. New methods like isothermal nucleic acid amplification tests offer a cost-effective solution for expanding COVID-19 testing.

FRONTIERS IN PUBLIC HEALTH (2021)

Add to Collection

Article Immunology

SARS-CoV-2 Wastewater Surveillance for Public Health Action

Jill S. McClary-Gutierrez et al.

Summary: Wastewater surveillance for SARS-CoV-2 has shown potential in complementing existing disease surveillance systems by correlating viral RNA concentrations in untreated sewage with reported case trends. Bridging the communication and knowledge gaps between researchers and public health responders is crucial for translating these measurements into useful public health tools. Addressing these barriers may pave the way for making this emerging public health tool a sustainable nationwide surveillance system.

EMERGING INFECTIOUS DISEASES (2021)

Add to Collection

Article Multidisciplinary Sciences

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Peng Zhou et al.

NATURE (2020)

Add to Collection

Editorial Material Engineering, Environmental

An Imperative Need for Research on the Role of Environmental Factors in Transmission of Novel Coronavirus (COVID-19)

Guangbo Qu et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2020)

Add to Collection

Editorial Material Engineering, Environmental

Can a Paper-Based Device Trace COVID-19 Sources with Wastewater-Based Epidemiology?

Kang Mao et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

CRISPR-Cas12-based detection of SARS-CoV-2

James P. Broughton et al.

NATURE BIOTECHNOLOGY (2020)

Add to Collection

Letter Medical Laboratory Technology

Antibody Profiles in Mild and Severe Cases of COVID-19

Zhi-Li Liu et al.

CLINICAL CHEMISTRY (2020)

Add to Collection

Editorial Material Engineering, Environmental

Wastewater-Based Epidemiology: Global Collaborative to Maximize Contributions in the Fight Against COVID-19

Aaron Bivins et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2020)

Add to Collection

Article Microbiology

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets

Chantal B. F. Vogels et al.

NATURE MICROBIOLOGY (2020)

Add to Collection

Review Biophysics

Detection of COVID-19: A review of the current literature and future perspectives

Tianxing Ji et al.

BIOSENSORS & BIOELECTRONICS (2020)

Add to Collection

Article Multidisciplinary Sciences

SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification

Brian A. Rabe et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Add to Collection

Article Multidisciplinary Sciences

The use of mobile phone data to inform analysis of COVID-19 pandemic epidemiology

Kyra H. Grantz et al.

NATURE COMMUNICATIONS (2020)

Add to Collection

Article Engineering, Biomedical

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

Maturada Patchsung et al.

NATURE BIOMEDICAL ENGINEERING (2020)

Add to Collection

Article Chemistry, Analytical

Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology

Bo Pang et al.

ANALYTICAL CHEMISTRY (2020)

Add to Collection

Article Biophysics

Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection

Yanju Chen et al.

BIOSENSORS & BIOELECTRONICS (2020)

Add to Collection

Review Chemistry, Analytical

Loop-mediated isothermal amplification (LAMP) - review and classification of methods for sequence-specific detection

Lisa Becherer et al.

ANALYTICAL METHODS (2020)

Add to Collection

Review Biotechnology & Applied Microbiology

CRISPR/Cas Systems towards Next-Generation Biosensing

Yi Li et al.

TRENDS IN BIOTECHNOLOGY (2019)

Add to Collection

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

Add to Collection

Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

Add to Collection

Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

Add to Collection

Article Chemistry, Analytical

Loop-mediated isothermal amplification using self-avoiding molecular recognition systems and antarctic thermal sensitive uracil-DNA-glycosylase for detection of nucleic acid with prevention of carryover contamination

Yi Wang et al.

ANALYTICA CHIMICA ACTA (2017)

Add to Collection

Article Veterinary Sciences

Investigation of false positives associated with loop-mediated isothermal amplification assays for detection of Toxoplasma gondii in archived tissue samples of captive felids

Essa Suleman et al.

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION (2016)

Add to Collection

Review Chemistry, Multidisciplinary

Isothermal Amplification of Nucleic Acids

Yongxi Zhao et al.

CHEMICAL REVIEWS (2015)

Add to Collection

Article Biochemical Research Methods

Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

Norihiro Tomita et al.

NATURE PROTOCOLS (2008)

Add to Collection

© Peeref 2019-2024. All rights reserved.