Evidence and Mechanisms of Selenate Reduction to Extracellular Elemental Selenium Nanoparticles on the Biocathode

Intracellular selenium nanoparticles (SeNPs) production is a roadblock to the recovery of selenium from biological water treatment processes because it is energy intensive to break microbial cells and then separate SeNPs. This study provided evidence of significantly more extracellular SeNP production on the biocathode (97-99%) compared to the conventional reactors (1-90%) using transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy. The cathodic microbial community analysis showed that relative abundance of Ospira oryzae, Desulfovibrio, Stenotrophomonas, and Rhodocyclaceae was <1% in the inoculum but enriched to 10-21% for each group when the bioelectrochemical reactor reached a steady state. These four groups of microorganisms simultaneously produce intracellular and extracellular SeNPs in conventional biofilm reactors per literature review but prefer to produce extracellular SeNPs on the cathode. This observation may be explained by the cellular energetics: by producing extracellular SeNPs on the biocathode, microbes do not need to transfer selenate and the electrons from the cathode into the cells, thereby saving energy. Extracellular SeNP production on the biocathode is feasible since we found high concentrations of C-type cytochrome, which is well known for its ability to transfer electrons from electrodes to microbial cells and reduce selenate to SeNPs on the cell membrane.

Automated summary

This study found that extracellular selenium nanoparticles (SeNPs) production on the biocathode was significantly higher compared to conventional reactors. Analysis of the microbial community on the cathode revealed an enrichment of Ospira oryzae, Desulfovibrio, Stenotrophomonas, and Rhodocyclaceae, which are known to produce both intracellular and extracellular SeNPs in conventional biofilm reactors. The observation suggests that microbes prefer to produce extracellular SeNPs on the cathode due to energy considerations.