Unveiling the the Mechanism of Urine Source Separation-Derived Pretreatment on Enhancing Short-Chain Fatty Acid Yields from Anaerobic Fermentation of Waste Activated Sludge

A novel strategy employing urine wastewater derived from source separation technology, to pretreat waste activated sludge (WAS) for promoting yields of short-chain fatty acids (SCFAs), has been proposed in this study. It was found experimentally that SCFA production could ascend up to 305.4 mg COD/g VSS (volatile suspended solids) with a urine volumetric proportion of 1:2 to the whole reaction system, being 8.8 times that produced in the control. Exploration of the mechanism indicated that WAS disintegration was significantly enhanced due to the synergistic effect of urea and free ammonia (FA). Degradation rates of model organic substrates and measurements of critical enzymatic activities demonstrated that hydrolysis and acidogenesis were inhibited under high urine content (urine proportion of 1:2), while not significantly affected under low urine content (i.e., 1:4), which might be attributed to metal ions existing in urine wastes alleviating the inhibition induced by FA. In contrast, methanogenesis was negatively suppressed by any urine concentration owing to its higher sensitivity to the environmental variations. Shift of microbial population further elucidated the abundance of hydrolytic and acidogenic microbes were enriched in the fermenters with urine addition. The findings provide a new thought for recovering resources from wastes, potentially reducing the pressure of sewage and sludge treatment in wastewater treatment plants.

Automated summary

A novel strategy using urine wastewater derived from source separation technology to pretreat waste activated sludge for increasing short-chain fatty acid (SCFA) yields has been proposed. The experimental results showed that SCFA production significantly increased when urine was added at a specific volumetric proportion. In addition, the presence of metal ions in urine waste was found to alleviate the inhibition effect on hydrolysis and acidogenesis.