Functional bacterial consortium responses to biochar and implications for BDE-47 transformation: Performance, metabolism, community assembly and microbial interaction

The influence of biochar on the biodegradation of persistent organic pollutants (POPs) has been extensively studied. However, the underlying mechanisms behind the response of functional microbial consortia to biochar remain poorly understood. Herein, we systematically explored the effect of biochar on 2,2'4,4 & PRIME;-tetrabrominated ether (BDE-47) biodegradation, and investigated the interaction and assembly mechanism of the functional bacterial consortium QY2. The results revealed that the biodegradation efficiency of QY2 for BDE-47 increased from 53.85% to 94.11% after the addition of biochar. Fluorescence excitation-emission matrix and electro-chemical analysis showed that biochar-attached biofilms were rich in redox-active extracellular polymeric substances (EPS, 3.03-fold higher than free cell), whose strong interaction with biochar facilitated the electron transfer of the biofilm, thus enhancing the debromination degradation of BDE-47. Meanwhile, the assembly model and molecular ecological networks analysis indicated that bacterial community assembly in biofilms was more driven by deterministic processes (environmental selection > 75.00%) upon biochar stimulation and exhibited closer interspecific cooperative interactions, leading to higher biodiversity and broader habitat niche breadth for QY2 in response to BDE-47 disturbance. Potential degraders (Methylobacterium, Sphingomonas, Microbacterium) and electrochemical bacteria (Ochrobactrum) were selectively enriched, whose role as keystone bacteria may be participated in biofilm formation and redox-active EPS secretion (r > 0.5, P < 0.05). These findings deepen the understanding of the mechanisms by which biochar promotes microbial degradation of PBDEs and provided a theoretical basis for better regulation of functional bacterial communities during envi-ronmental remediation.

Automated summary

This study investigated the effect of biochar on the biodegradation of BDE-47 and explored the interaction and assembly mechanism of the functional bacterial consortium QY2. The results showed that the addition of biochar significantly increased the biodegradation efficiency of BDE-47 by QY2. The presence of biochar promoted electron transfer in the biofilm through the strong interaction with redox-active extracellular polymeric substances (EPS), leading to enhanced debromination degradation of BDE-47. The assembly model and molecular ecological networks analysis revealed that the bacterial community assembly in biofilms was mainly driven by deterministic processes, resulting in higher biodiversity and broader habitat niche breadth for QY2 in response to BDE-47 disturbance.