Responses to waterlogging stress in quinoa seedlings based on metabolomic and transcriptomic analysis

Quinoa is a dicotyledonous annual herb has cold and drought resistance. However, there is little research on quinoa under waterlogging stress. We analyzed the metabolomics and transcriptomics of Dianli-1299 (water-logging-resistant strain), Dianli-60, and Yuncaili-2 (non-waterlogging-resistant strains) quinoa seedlings. Through metabolome, we detected a total of 1058 metabolites. And 337, 300, and 386 differential metabolites were found in Dianli-1299, Dianli-60, and Yuncaili-2, respectively. Transcriptome analysis showed increased expression of saccharides and alcohols related genes in Dianli-1299 compared to Dianli-60 and Yuncaili-2. Gene-LOC110708270 and gene-LOC110681806, which were related to UTP-glucose-1-phosphate uridylyltransferase (UGP2), were significantly expressed in Dianli-1299 treatment group, and the regulated galactinol content was significantly accumulated in Dianli-1299 treatment group. Gene-LOC110736796 in Dianli-60 treatment group was significantly higher than that in Dianli-1299 treatment group, and the D-Ribose-5 P content, which is regulated by gene-LOC110736796 was lower in Dianli-60 treatment group than that in Dianli-1299 treatment group. In Dianli-1299, the raffinose content regulated by gene-LOC110707988 and gene-LOC110725594 was higher than that in Dianli-60 and Yuncaili-2. The correlation coefficients of 18 differential metabolites and differential genes were greater than 0.9, which suggested that these genes and metabolites might be key factors for quinoa to cope with waterlogging stress. Our results may provide a basis for breeding and identifying waterlogging-resistant quinoa varieties.

Automated summary

In this study, metabolomics and transcriptomics analysis were conducted to investigate the differences between waterlogging-resistant and non-waterlogging-resistant quinoa varieties. The results showed that waterlogging-resistant varieties had increased expression of genes related to saccharides and alcohols, as well as the ability to regulate galactinol and raffinose content. Compared to non-waterlogging-resistant varieties, waterlogging-resistant varieties exhibited better adaptation to waterlogging stress, which may provide a basis for breeding and identifying waterlogging-resistant quinoa varieties.